The word Chromatography is derived from the greek word Khromatos-graphos meaning colour
writing. It was first introduces by Mikhail Tswett. Chromatography is an important technique for the
separation, isolation and identification of the constituents of a mixture. It is a a non-destructive physical
method of separation in which the components to be separated are distributed between two phases- phase
of large surface area fixed in place in a column or on a flat solid surface known as stationary phase and
a fluid phase which percolates through or along the stationary phase known as mobile phase.
INTRODUCTION PRINCIPLE AND CLASSIFICCATION
ADSORPTION AND PARTITION CHROMATOGRAPHY
ION EXCHANGE CHROMATOGRAPHY
Electrolytic conductivity, molar conductivity – Variation of molar conductivitywith concentration. Kohlrausch’s law – Applications
relation with ion conductivity, influence of temperature on ionconductivity, ion conductivity and viscosity – Walden’s rule. Abnormal ion conductivity of hydrogen and hydroxyl ions.
SIZE EXCLUSION CHROMATOGRAPHY